The Human FETUA (Fetuin-A) ELISA Kit is specifically designed for the precise measurement of fetuin-A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and trustworthy results for various research purposes.Fetuin-A is a key protein that plays a vital role in regulating calcium ion concentration and inhibiting calcification in the body. It is involved in various physiological processes, including bone metabolism, insulin sensitivity, and inflammation. Abnormal fetuin-A levels have been linked to conditions such as diabetes, cardiovascular disease, and chronic kidney disease, making it a valuable biomarker for studying these disorders and exploring potential treatment options.Overall, the Human FETUA (Fetuin-A) ELISA Kit is a valuable tool for researchers looking to investigate the role of fetuin-A in health and disease, providing accurate and reliable data for groundbreaking discoveries in the field of biomedical research.
Product Name:
Human FETUA (Fetuin A) ELISA Kit
SKU:
HUES01601
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
5.63 ng/mL
Detection range:
9.38-600 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
