Human Fatty Acid Transport Protein 1 (FATP1) ELISA Kit
The Human Fatty Acid Transport Protein 1 (FATP1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of FATP1 levels in human serum, plasma, and cell culture supernatants. This kit is an essential tool for researchers studying lipid metabolism, obesity, and related disorders.FATP1 is a key protein involved in the transport and metabolism of fatty acids, playing a crucial role in maintaining lipid homeostasis in cells.
Dysregulation of FATP1 has been linked to various metabolic diseases such as diabetes, cardiovascular diseases, and non-alcoholic fatty liver disease.With its high precision and reproducibility, the FATP1 ELISA Kit offers researchers a reliable method for quantifying FATP1 levels in biological samples, aiding in the understanding of lipid metabolism and the development of targeted therapies for metabolic disorders.
Product Name:
Human Fatty Acid Transport Protein 1 (FATP1) ELISA Kit
Product Code:
HUDL01023
Size:
96 Assays
Target:
FATP1
Synonyms:
SLC27A1, ACSVL5, FATP, Long-Chain Fatty Acid Transport Protein 1, Solute Carrier Family 27 Member 1,Fatty Acid Transporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
