Human DNA Damage Response Array (27 targets) (SARB0106)

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SKU:
SARB0106

Description

system_update_altDatasheet
Product Sku: SARB0106
Size: 2, 4, or 8
Species Detected: Human
Number of Targets Detected: 27
Gene Symbols: ABL1, APEX1, ATR, BRCA1, BRCA2, CCNB1, CDC25A, CDK1, CDK7, CDKN1A| CHEK1, CHEK2, DDIT3, ERCC1, MDM2, MGMT, MSH2, NBN, OPTN, PARP1, PLK1, PPM1D, PRKDC, RAD17, TP53, XRCC5, XRCC6
Compatible Sample Types: Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Chemiluminescence
Quantitative/Semi-Quantitative: Semi-Quantitative
Solid Support: Membrane
Suggested Application(s): Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process Process
Storage/Stability: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human DNA Damage Response Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. Block membranes
2. Incubate with Sample
3. Incubate with Biotinylated Detection Antibody Cocktail
4. Incubate with HRP-Conjugated Streptavidin
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis
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Additional Information

Reactivity:
Human
Applications:
Multiplex Array
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