Human Cytokine Array (80 targets) (SARB0040)
- SKU:
- SARB0040
- Product Type:
- Protein Array
- Analytes:
- Angiogenin
- Analytes:
- BDNF
- Analytes:
- BLC (CXCL13)
- Analytes:
- CK beta 8-1 (CCL23)
- Analytes:
- EGF
- Analytes:
- ENA-78 (CXCL5)
- Analytes:
- Eotaxin-1 (CCL11)
- Analytes:
- Eotaxin-2 (MPIF-2/CCL24)
- Analytes:
- Eotaxin-3 (CCL26)
- Analytes:
- FGF-4
- Analytes:
- FGF-6
- Analytes:
- FGF-7 (KGF)
- Analytes:
- FGF-9
- Analytes:
- Flt-3 Ligand
- Analytes:
- Fractalkine (CX3CL1)
- Analytes:
- GCP-2 (CXCL6)
- Analytes:
- GCSF
- Analytes:
- GDNF
- Analytes:
- GM-CSF
- Analytes:
- GRO alpha (CXCL1)
- Analytes:
- GRO alpha/beta/gamma
- Analytes:
- HGF
- Analytes:
- I-309 (TCA-3/CCL1)
- Analytes:
- IFN-gamma
- Analytes:
- IGF-1
- Analytes:
- IGFBP-1
- Analytes:
- IGFBP-2
- Analytes:
- IGFBP-3
- Analytes:
- IGFBP-4
- Analytes:
- IL-1 alpha (IL-1 F1)
- Analytes:
- IL-1 beta (IL-1 F2)
- Analytes:
- IL-2
- Analytes:
- IL-3
- Analytes:
- IL-4
- Analytes:
- IL-5
- Analytes:
- IL-6
- Analytes:
- IL-7
- Analytes:
- IL-8 (CXCL8)
- Analytes:
- IL-10
- Analytes:
- IL-12 p40/p70
- Analytes:
- IL-13
- Analytes:
- IL-15
- Analytes:
- IL-16
- Analytes:
- IP-10 (CXCL10)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0040 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 80 |
| Gene Symbols: | ANG, BDNF, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20|CCL22, CCL23, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CSF1, CSF2, CSF3, CX3CL1, CXCL1, CXCL10, CXCL12, CXCL13, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, FGF4, FGF6, FGF7, FGF9, FLT3LG, GDNF, HGF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IL10, IL12A, IL13, IL15, IL16, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LIF, LTA, MIF, NTF3, NTF4, OSM, PDGFB, PGF, PPBP, SPP1, TGFB1, TGFB2, TGFB3, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFSF14, TPO, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| EGF
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| FGF-4
|
FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GCSF
| GDNF
| GM-CSF
| GRO alpha (CXCL1)
|
GRO alpha/beta/gamma
| HGF
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-12 p40/p70
|
IL-13
| IL-15
| IL-16
| IP-10 (CXCL10)
| Leptin
|
LIF
| LIGHT (TNFSF14)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
| MIF
| MIG (CXCL9)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
NT-4
| Oncostatin M
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-BB
| PLGF
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 2
| TGF beta 3
| Thrombopoietin (TPO)
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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