Human Cytokine Array (49 targets) (SARB0032)
- SKU:
- SARB0032
- Product Type:
- Protein Array
- Analytes:
- 4-1BB (TNFRSF9/CD137)
- Analytes:
- ACE-2
- Analytes:
- Alpha-fetoprotein
- Analytes:
- Angiopoietin-1
- Analytes:
- Angiostatin
- Analytes:
- ANGPTL4
- Analytes:
- Beta-2 Microglobulin
- Analytes:
- BCMA (TNFRSF17)
- Analytes:
- beta IG-H3
- Analytes:
- CA125
- Analytes:
- CA15-3
- Analytes:
- CA19-9
- Analytes:
- CA9
- Analytes:
- Cathepsin S
- Analytes:
- HCC-1 (CCL14)
- Analytes:
- 6Ckine (CCL21)
- Analytes:
- CD23
- Analytes:
- CD40 Ligand (TNFSF5)
- Analytes:
- CEA
- Analytes:
- CEACAM-1
- Analytes:
- Cripto-1
- Analytes:
- CRP (C-Reactive Protein)
- Analytes:
- DAN
- Analytes:
- Decorin
- Analytes:
- DKK-1
- Analytes:
- Dkk-3
- Analytes:
- Dkk-4
- Analytes:
- CD26 (DPPIV)
- Analytes:
- E-Cadherin
- Analytes:
- EDA-A2
- Analytes:
- EG-VEGF (PK1)
- Analytes:
- TROP1 (EpCAM)
- Analytes:
- ErbB2
- Analytes:
- Erythropoietin R
- Analytes:
- FSH
- Analytes:
- HB-EGF
- Analytes:
- hCG intact
- Analytes:
- HVEM (TNFRSF14)
- Analytes:
- IL-13 R1
- Analytes:
- IL-17B
- Analytes:
- IL-17C
- Analytes:
- IL-17F
- Analytes:
- IL-17 RA
- Analytes:
- Procalcitonin
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0032 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 49 |
| Gene Symbols: | ACE2, AFP, ANGPT1, ANGPTL4, B2M, CA (19-9), CA9, CALCA, CCL14, CCL21|CD40LG, CDH1, CEACAM1, CEACAM5, CGA, CGB, CRP, CTSS, DCN, DKK1, DKK3, DKK4, DPP4, EDA, EPCAM, EPOR, ERBB2, FCER2, FSHB, HBEGF, IL13RA1, IL17B, IL17C, IL17F, IL17RA, KLK3, MUC1, MUC16, PARN, PLG, PROK1, RPS27A, S100B, SHH, TDGF1, TG, TGFBI, TNFRSF14, TNFRSF17, TNFRSF9, TNFSF9 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| ACE-2
| Alpha-fetoprotein
| Angiopoietin-1
| Angiostatin
|
ANGPTL4
| Beta-2 Microglobulin
| BCMA (TNFRSF17)
| beta IG-H3
| CA125
|
CA15-3
| CA19-9
| CA9
| Cathepsin S
| HCC-1 (CCL14)
|
6Ckine (CCL21)
| CD23
| CD40 Ligand (TNFSF5)
| CEA
| CEACAM-1
|
Cripto-1
| CRP (C-Reactive Protein)
| DAN
| Decorin
| DKK-1
|
Dkk-3
| Dkk-4
| CD26 (DPPIV)
| E-Cadherin
| EDA-A2
|
EG-VEGF (PK1)
| TROP1 (EpCAM)
| ErbB2
| Erythropoietin R
| FSH
|
HB-EGF
| hCG intact
| HVEM (TNFRSF14)
| IL-13 R1 | IL-17B
|
IL-17C
| IL-17F
| IL-17 RA
| Procalcitonin
| PSA-free
|
S100 B
| Sonic Hedgehog N-Terminal (Shh-N)
| Thyroglobulin
| Ubiquitin+1
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C10 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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