Human Cytokine Array (42 targets) (SARB0037)

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SKU:
SARB0037
Product Type:
Protein Array
Analytes:
ENA-78 (CXCL5)
Analytes:
GCSF
Analytes:
GM-CSF
Analytes:
GRO alpha/beta/gamma
Analytes:
GRO alpha (CXCL1)
Analytes:
I-309 (TCA-3/CCL1)
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-2
Analytes:
IL-3
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-6
Analytes:
IL-7
Analytes:
IL-8 (CXCL8)
Analytes:
IL-10
Analytes:
IL-12 p40/p70
Analytes:
IL-13
Analytes:
IL-15
Analytes:
IFN-gamma
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-2 (CCL8)
Analytes:
MCP-3 (MARC/CCL7)
Analytes:
M-CSF
Analytes:
MDC (CCL22)
Analytes:
MIG (CXCL9)
Analytes:
MIP-1 delta (CCL15)
Analytes:
RANTES (CCL5)
Analytes:
SCF
Analytes:
SDF-1 alpha (CXCL12 alpha)
Analytes:
TARC (CCL17)
Analytes:
TGF beta 1
Analytes:
TNF alpha
Analytes:
TNF beta (TNFSF1B)
Analytes:
EGF
Analytes:
IGF-1
Analytes:
Angiogenin
Analytes:
Oncostatin M
Analytes:
Thrombopoietin (TPO)
Analytes:
VEGF-A
Analytes:
PDGF-BB
Analytes:
Leptin
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0037
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:42
Gene Symbols:ANG, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CSF1|CSF2, CSF3, CXCL1, CXCL12, CXCL2, CXCL3, CXCL5, CXCL8, CXCL9, EGF, IFNG, IGF1, IL10, IL12A, IL13, IL15, IL1A, IL1B, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LTA, OSM, PDGFB, TGFB1, THPO, TNF, TPO, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
ENA-78 (CXCL5)
  • Gene Symbol: CXCL5
  • Gene ID: 6374
  • Accession #: P42830
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
GRO alpha/beta/gamma
  • Gene Symbol: CXCL1/CXCL2/CXCL3
  • Gene ID: 2919/2920/2921
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: 2919
  • Accession #: P09341
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
IL-8 (CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Gene ID: N/A
  • Accession #: P29459
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
MDC (CCL22)
  • Gene Symbol: CCL22
  • Gene ID: 6367
  • Accession #: O00626
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
MIP-1 delta (CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
SCF
  • Gene Symbol: KITLG
  • Gene ID: 4254
  • Accession #: P21583
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: 6361
  • Accession #: Q92583
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
Angiogenin
  • Gene Symbol: ANG
  • Gene ID: 283
  • Accession #: P03950
Oncostatin M
  • Gene Symbol: OSM
  • Gene ID: 5008
  • Accession #: P13725
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: 7066
  • Accession #: P40225
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
Leptin
  • Gene Symbol: LEP
  • Gene ID: 3952
  • Accession #: P41159
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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