Human Cytokine Array (174 targets) (SARB0036)
- SKU:
- SARB0036
- Product Type:
- Protein Array
- Analytes:
- Adiponectin (ACRP30)
- Analytes:
- Activin A
- Analytes:
- AgRP
- Analytes:
- ALCAM (CD166)
- Analytes:
- Amphiregulin
- Analytes:
- Angiogenin
- Analytes:
- Angiopoietin-2
- Analytes:
- Axl
- Analytes:
- CD80 (B7-1)
- Analytes:
- BDNF
- Analytes:
- bFGF
- Analytes:
- BLC (CXCL13)
- Analytes:
- BMP-4
- Analytes:
- BMP-5
- Analytes:
- BMP-6
- Analytes:
- BMP-7
- Analytes:
- beta-NGF
- Analytes:
- Betacellulin (BTC)
- Analytes:
- Cardiotrophin-1 (CT-1)
- Analytes:
- CCL28 (MEC)
- Analytes:
- CD14
- Analytes:
- Ck beta 8-1 (CCL23)
- Analytes:
- CNTF
- Analytes:
- CTACK (CCL27)
- Analytes:
- CXCL16
- Analytes:
- DR6 (TNFRSF21)
- Analytes:
- Dtk
- Analytes:
- EGF
- Analytes:
- EGFR
- Analytes:
- ENA-78 (CXCL5)
- Analytes:
- Endoglin (CD105)
- Analytes:
- Eotaxin-1 (CCL11)
- Analytes:
- Eotaxin-2 (MPIF-2/CCL24)
- Analytes:
- Eotaxin-3 (CCL26)
- Analytes:
- ErbB3
- Analytes:
- E-Selectin
- Analytes:
- Fas (TNFRSF6/Apo-1)
- Analytes:
- Fas Ligand (TNFSF6)
- Analytes:
- FGF-4
- Analytes:
- FGF-6
- Analytes:
- FGF-7 (KGF)
- Analytes:
- FGF-9
- Analytes:
- Flt-3 Ligand
- Analytes:
- Fractalkine (CX3CL1)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0036 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 174 |
| Gene Symbols: | ADIPOQ, AGRP, ALCAM, ANG, ANGPT2, AREG, AXL, BDNF, BMP4, BMP5|BMP6, BMP7, BTC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD14, CD80, CDH5, CNTF, CSF1, CSF1R, CSF2, CSF3, CTF1, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CXCL9, EGF, EGFR, ENG, ERBB3, FAS, FASLG, FGF2, FGF4, FGF6, FGF7, FGF9, FIGF, FLT3LG, FLT4, GDNF, HGF, ICAM1, ICAM2, ICAM3, IFNG, IGF1, IGF1R, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP6, IL10, IL10RB, IL11, IL12A, IL12B, IL13, IL13RA2, IL15, IL16, IL17A, IL18BP, IL18RAP, IL1A, IL1B, IL1R1, IL1R2, IL1RL1, IL1RN, IL2, IL21R, IL2RA, IL2RB, IL2RG, IL3, IL4, IL5, IL5RA, IL6, IL6R, IL6ST, IL7, IL9, INHBA, KDR, KIT, KITLG, LEP, LEPR, LIF, LTA, MIF, MMP1, MMP13, MMP3, MMP9, MST1, NGF, NGFR, NTF3, NTF4, OSM, PDGFA, PDGFB, PDGFRA, PDGFRB, PECAM1, PGF, PLAUR, PPBP, PRL, SELE, SELL, SIGLEC5, TEK, TGFA, TGFB1, TGFB2, TGFB3, THPO, TIE1, TIMP1, TIMP2, TIMP4, TNF, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFRSF21, TNFSF14, TNFSF18, TPO, TYRO3, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| Activin A
| AgRP
| ALCAM (CD166)
| Amphiregulin
|
Angiogenin
| Angiopoietin-2
| Axl
| CD80 (B7-1)
| BDNF
|
bFGF
| BLC (CXCL13)
| BMP-4
| BMP-5
| BMP-6
|
BMP-7
| beta-NGF
| Betacellulin (BTC)
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
|
CD14
| Ck beta 8-1 (CCL23)
| CNTF
| CTACK (CCL27)
| CXCL16
|
DR6 (TNFRSF21)
| Dtk
| EGF
| EGFR
| ENA-78 (CXCL5)
|
Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| ErbB3
|
E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| FGF-4
| FGF-6
|
FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
|
GCSF
| GDNF
| GITR (TNFRSF18)
| GITR Ligand (TNFSF18)
| GM-CSF
|
GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| I-309 (TCA-3/CCL1)
|
ICAM-1 (CD54)
| ICAM-2 (CD102)
| ICAM-3 (CD50)
| IFN-gamma
| IGFBP-1
|
IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
|
IGF-1 R
| IGF-2
| IL-1 R2
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-10
| IL-10 R beta
| IL-11
| IL-12 p40
| IL-12 p70
|
IL-13
| IL-13 R alpha 2
| IL-15
| IL-16
| IL-17A
|
IL-18 BP alpha
| IL-18 R beta (AcPL)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
IL-2
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-2 R alpha
| IL-21 R
|
IL-3
| IL-4
| IL-5
| IL-5 R alpha
| IL-6
|
IL-6 R
| IL-7
| IL-8 (CXCL8)
| IL-9
| IP-10 (CXCL10)
|
I-TAC (CXCL11)
| LAP/TGF beta 1
| Leptin
| Leptin R
| LIF
|
Light (TNFSF14)
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
|
MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| M-CSF R
| MDC (CCL22)
|
MIF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
|
MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
| MMP-1
| MMP-13
| MMP-3
|
MMP-9
| MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| NGFR (TNFRSF16)
|
NT-3
| NT-4
| Oncostatin M
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-AA
| PDGF R alpha
| PDGF R beta
| PDGF-AB
| PDGF-BB
|
PECAM-1 (CD31)
| PLGF
| Prolactin
| RANTES (CCL5)
| SCF
|
SCF R (CD117/c-kit)
| SDF-1 alpha (CXCL12 alpha)
| SDF-1 beta (CXCL12 beta)
| gp130
| Siglec-5 (CD170)
|
TNF RII (TNFRSF1B)
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TECK (CCL25)
| TGF beta 2
|
TGF alpha
| TGF beta 3
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-1
|
Tie-2
| TIMP-1
| TIMP-2
| TIMP-4
| TNF alpha
|
TNF beta (TNFSF1B)
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VE-Cadherin (CDH5)
|
VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C2000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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