The Human Cyt-C (Cytochrome C) ELISA Kit is specifically designed for the precise measurement of cytochrome C levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Cytochrome C is a key component in the electron transport chain, playing a vital role in mitochondrial function and cell apoptosis. Dysregulation of cytochrome C has been linked to various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a biomarker for studying disease mechanisms and potential therapeutic interventions. With its reliable performance and ease of use, the Human Cyt-C (Cytochrome C) ELISA Kit is an invaluable tool for researchers looking to investigate the role of cytochrome C in health and disease.
Product Name:
Human Cyt-C (Cytochrome C) ELISA Kit
SKU:
HUES01343
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
