Human CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
The Human CTX-II (Cross-linked C-telopeptide of Type II Collagen) ELISA Kit is specifically designed for the accurate measurement of CTX-II levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are perfect for a variety of research applications.CTX-II is a key biomarker associated with the degradation of type II collagen in cartilage, making it a valuable marker for studying conditions such as osteoarthritis and rheumatoid arthritis. By measuring CTX-II levels, researchers can gain insights into cartilage degradation, disease progression, and potential therapeutic interventions.Overall, the Human CTX-II ELISA Kit provides researchers with a reliable tool for studying cartilage degradation and monitoring disease progression, offering valuable insights for the development of new treatments for conditions related to type II collagen degradation.
Product Name:
Human CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
SKU:
HUES01971
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
