Human CKLFSF6/Chemokine Like Factor Superfamily 6 ELISA Kit (HUFI02324)
- SKU:
- HUFI02324
- Product Type:
- ELISA Kit
- Uniprot:
- Q9NX76
- ELISA Type:
- Sandwich
- Synonyms:
- CKLFSF6, CKLF-like MARVEL transmembrane domain-containing protein 6, CMTM6
- Reactivity:
- Human
- Research Area:
- Signal Transduction
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.094 ng/mL
- Range:
- 0.156-10 ng/mL
Description
Human CKLFSF6/Chemokine Like Factor Superfamily 6 ELISA Kit
The Human CKLFSF6 (Chemokine-Like Factor Superfamily 6) ELISA Kit is specially designed for the precise measurement of CKLFSF6 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures dependable and consistent results, making it an invaluable tool for a variety of research purposes. CKLFSF6 is a significant molecule that belongs to the chemokine-like factor superfamily and plays a key role in immune responses and inflammatory processes.
It is implicated in various diseases such as autoimmune disorders, inflammatory conditions, and cancer, making it a crucial biomarker for investigating these diseases and exploring potential treatment options. Overall, the Human CKLFSF6 ELISA Kit offers researchers a reliable and efficient method for quantifying CKLFSF6 levels, providing valuable insights into its biological functions and clinical implications.
| Product Name: | Human CKLFSF6/Chemokine Like Factor Superfamily 6 ELISA Kit |
| SKU: | HUFI02324 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.094 ng/mL |
| Range: | 0.156-10 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
| ||||||||||||||||
| Recovery: |
|
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
Related Products
Human CKLFSF6 (Chemokine Like Factor Superfamily 6) ELISA Kit (HUES01862)
Human Signal Transduction ELISA Kits
Rat CKLFSF6/Chemokine Like Factor Superfamily 6 ELISA Kit (RTFI00669)
Rat Immunology ELISA Kits 2
Rat Chemokine Like Factor Superfamily 5/CKLFSF5 ELISA Kit (RTFI00668)
Rat Immunology ELISA Kits 2
Mouse CKLFSF4/Chemokine Like Factor Superfamily 4 ELISA Kit (MOFI00723)
Mouse Cell Signalling ELISA Kits 1