The Human CHGA (Chromogranin A) ELISA Kit is specially designed for the accurate measurement of Chromogranin A levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it an indispensable tool for a wide range of research applications.Chromogranin A is a vital protein that plays a key role in various physiological processes, including neurotransmitter release and hormone secretion. It is implicated in a number of diseases such as neuroendocrine tumors, pheochromocytoma, and heart failure, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.This Human CHGA ELISA Kit provides researchers with a powerful tool to accurately quantify Chromogranin A levels, enabling a deeper understanding of its role in health and disease. Order now and unlock the potential of Chromogranin A in your research endeavors.
Product Name:
Human CHGA (Chromogranin A) ELISA Kit
SKU:
HUES01884
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
