Human CENPA Recombinant Protein (RPPB3144)

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SKU:
RPPB3144
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49450

Description

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Product Name:Human CENPA Recombinant Protein
Product Code:RPPB3144
Size:20µg
Species:Human
Target:CENPA
Synonyms:Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered liquid formulation.
Formulation:CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients.

CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

UniProt Protein Function:CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: chromosome, pericentric region; condensed nuclear chromosome, pericentric region; cytosol; nucleoplasm; nucleus

Molecular Function:chromatin binding; protein binding

Biological Process: DNA replication-independent nucleosome assembly at centromere; establishment of mitotic spindle orientation; kinetochore assembly; sister chromatid cohesion

NCBI Summary:Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]
UniProt Code:P49450
NCBI GenInfo Identifier:1345726
NCBI Gene ID:1058
NCBI Accession:P49450.1
UniProt Secondary Accession:P49450,Q53T74, Q9BVW2, D6W544,
UniProt Related Accession:P49450
Molecular Weight:13,001 Da
NCBI Full Name:Histone H3-like centromeric protein A
NCBI Synonym Full Names:centromere protein A
NCBI Official Symbol:CENPA��
NCBI Official Synonym Symbols:CenH3; CENP-A��
NCBI Protein Information:histone H3-like centromeric protein A
UniProt Protein Name:Histone H3-like centromeric protein A
UniProt Synonym Protein Names:Centromere autoantigen A; Centromere protein A; CENP-A
Protein Family:Histone H3-like centromeric protein
UniProt Gene Name:CENPA��
UniProt Entry Name:CENPA_HUMAN
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