Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Product Name:
Human CEACAM6/CD66c Recombinant Protein (RPES0657)
Product Code:
RPES0657
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CD66c,CEA,CEAL,NCA
Accession:
NP_002474.3
Sequence:
Met 1-Gly 320
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 µg/mL (100 ?l/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CEACAM6 (NP_002474.3) (Met 1-Gly 320) with a C-terminal polyhistidine tag was expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
32.6 kDa
AP Mol Mass:
45-55 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
