Antibody Name:	Human CD47 Monoclonal Antibody (Alexa Fluor 488 Conjugated)
Antibody Code:	AGEL0254
Size:	50 Tests
Clone:	CC2C6D4
Synonyms:	Leukocyte surface antigen CD47, Cd47, Integrin-associated protein, IAP
Host Species:	Mouse
Species Reactivity:	Human
Applications:	FC

Concentration:	5uL
Isotype:	IgG1
Conjugation:	Alexa Fluor 488
Applications:	FC
Recommended Dilution:	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:	PBS with 0.05% Proclin300, 1% BSA
Storage:	Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.

Gene ID:	961
Uniprot:	Q08722
Background:	CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.

Mouse CD19 Monoclonal Antibody (FITC Conjugated)

Human peripheral blood lymphocytes are stained with Anti-Human CD47 Monoclonal Antibody (Alexa Fluor 488 Conjugated)(filled gray histogram). Unstained lymphocytesÂ (empty black histogram) are used as control.

Antibodies

Human/Mouse/Rat CD47 Monoclonal Antibody [MIAP410](AGEL0107)

Human/Mouse/Rat CD47 Monoclonal Antibody (Biotin Conjugated) [MIAP410](AGEL0108)

Human CD47 Monoclonal Antibody [CC2C6D4](AGEL0249)

Human CD47 Monoclonal Antibody (Biotin Conjugated) [CC2C6D4](AGEL0250)

Human CD47 Monoclonal Antibody (FITC Conjugated) [CC2C6D4](AGEL0251)