Human CD31/PECAM1 Recombinant Protein (RPES0218)
- SKU:
- RPES0218
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
Human CD31/PECAM1 Recombinant Protein
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
| Product Name: | Human CD31/PECAM1 Recombinant Protein (RPES0218) |
| Product Code: | RPES0218 |
| Size: | 100µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA, PECA1, CD31, PECAM1 |
| Accession: | EAW94208.1 |
| Sequence: | Met 1-Lys 601 |
| Fusion tag: | C-His |
| Activity: | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to CD31-His coated plates (10µg/mL, 100 ?L/well) in the presence of 20 ng/mL PMA, approximately 35-45% will adhere after 30 minutes at 37?. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human CD31 (EAW94208.1) was expressed with a polyhistidine tag at the C-terminus. |
| Purity: | > 97 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 66 kDa |
| AP Mol Mass: | 100-110 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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