Description
Antibody Name: | Human CD25 Monoclonal Antibody (PE Conjugated) |
Antibody Code: | AGEL2017 |
Size: | 50 Tests |
Clone: | BC96 |
Synonyms: | Interleukin-2 receptor subunit alpha,IL2RA,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,p55 |
Host Species: | Mouse |
Species Reactivity: | Human |
Applications: | FC |
Concentration: | 5uL |
Isotype: | IgG2a,k |
Conjugation: | PE |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Gene ID: | 3559 |
Uniprot: | P01589 |
Background: | CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex. |
Human peripheral blood lymphocytes are stained with Anti-Human CD25 Monoclonal Antibody(PE Conjugated) and Anti-Human CD4 Monoclonal Antibody(APC Conjugated)(Left). lymphocytes stained with Anti-Human CD4 Monoclonal Antibody(APC Conjugated) and Mouse IgG2a,k Isotype Control(PE Conjugated)(Right) are used as control. |