CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD150 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV). As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of IFN-&gamma in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.
Product Name:
Human CD150/SLAMF1 Recombinant Protein (RPES0541)
Product Code:
RPES0541
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Signaling Lymphocytic Activation Molecule, CDw150, IPO-3, CD150, SLAMF1, SLAM
Accession:
NP_003028.1
Sequence:
Met 1-Pro 258
Fusion tag:
C-His
Activity:
Measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SLAM (NP_003028.1) precursor (Met 1-Pro 258) with a C-terminal polyhistidine tag was expressed.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
25.8 kDa
AP Mol Mass:
45-50 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetHuman CD150/SLAMF1 Recombinant Protein SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are like
