The Human Caspase-4 ELISA Kit is specially designed for the precise measurement of Caspase-4 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it the perfect choice for a variety of research purposes.Caspase-4 is a key enzyme in the inflammatory response pathway, playing a critical role in immune responses and cellular stress. Dysregulation of Caspase-4 has been linked to various diseases including sepsis, inflammatory bowel disease, and neurodegenerative disorders, highlighting its importance as a biomarker for understanding and treating these conditions.Order the Human Caspase-4 ELISA Kit today and unlock new insights into the function and implications of Caspase-4 in health and disease.
Product Name:
Human CASP4 (Caspase 4) ELISA Kit
SKU:
HUES01819
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
