Human C1QTNF1/Complement C1q tumor necrosis factor-related protein 1 ELISA Kit (HUFI01337)

Human C1QTNF1/Complement C1q tumor necrosis factor-related protein 1 ELISA Kit

The Human C1QTNF1 (Complement C1q Tumor Necrosis Factor-Related Protein 1) ELISA Kit is specifically designed for the precise measurement of C1QTNF1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. C1QTNF1 is a key protein involved in various physiological processes, including inflammation, adipogenesis, and energy metabolism.

Dysregulation of C1QTNF1 has been linked to metabolic disorders, cardiovascular diseases, and inflammatory conditions, making it an important biomarker for understanding these diseases and developing potential therapeutic interventions. With its reliable performance and broad applicability, the Human C1QTNF1 ELISA Kit is an essential tool for researchers studying the role of C1QTNF1 in health and disease.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.