Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit
The Human BCL-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit is specially designed for the precise measurement of BCL-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results, and is well-suited for various research applications.BCL-2 is a key regulator of apoptosis, playing a crucial role in cell survival and resistance to programmed cell death. Dysregulation of BCL-2 has been implicated in various diseases, including leukemia, lymphoma, and other cancers, making it a valuable biomarker for studying these conditions and potentially developing new treatments.Order the Human BCL-2 ELISA Kit today to advance your research in cancer biology, immunology, and molecular medicine.
Product Name:
Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit
SKU:
HUES01400
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
