Epigenetics & Nuclear Signaling Recombinant Proteins

Human ATF1 Recombinant Protein (RPES1918)

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RPES1918

Description

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Human ATF1 Recombinant Protein

Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Product Name:Human ATF1 Recombinant Protein (RPES1918)
Product Code:RPES1918
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Cyclic AMP-dependent transcription factor ATF-1,cAMP-dependent transcription factor ATF-1,Activating transcription factor 1,
Accession:P18846
Sequence:Met 1-Val271
Fusion tag:C-6His
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Activating Transcription Factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val271 is expressed with a 6His tag at the C-terminus.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:30.3 kDa
AP Mol Mass:32 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Research Area:
Epigenetics & Nuclear Signaling
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