Human ApoH PharmaGenie ELISA Kit (SBRS0278)
- SKU:
- SBRS0278
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Human ApoH PharmaGenie ELISA Kit (SBRS0278)
| Product SKU: | SBRS0278 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, Adiponectin, Adipsin, Albumin, Angiogenin, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, B2M, CD14, Clusterin, CRP, Cystatin C, ECM-1, Fetuin A, Lipocalin-2, LYVE-1, MBL, NAP-2, PF4, RBP4, SAA, Serpin A4, TfR, TSP-1, Transferrin, Vitronectin, vWF. |
| Uniprot: | P02749 |
| Gene ID: | 350 |
| Gene Names: | APOH / B2G1 |
| Synonyms: | Beta-2-glycoprotein 1 (APC inhibitor) (Activated protein C-binding protein) (Anticardiolipin cofactor) (Apolipoprotein H) (Apo-H) (Beta-2-glycoprotein I) (B2GPI) (Beta(2)GPI) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 30 pg/ml - 6000 pg/ml |
| Sensitivity: | 30 pg/ml |
| Recommended Dilution: | 1,000,000 fold |
| Storage/Stability: | May be stored for up to 6 months at 2 to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human ApoH PharmaGenie ELISA Kit (SBRS0278) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
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