Human ANXA4 ELISA Kit (HUFI01594)
- SKU:
- HUFI01594
- Product Type:
- ELISA Kit
- Uniprot:
- P09525
- ELISA Type:
- Sandwich
- Synonyms:
- ANXA4, Annexin A4, AIV, Annexin IV, ANX4, Lipocortin IV, PIG28, ZAP36, annexin IV, placental anticoagulant protein II, Annexin-4, CarbohydRate-binding protein p33, p41, Chromobindin-4, chromobindin-4, 35-beta calcimedin, Endonexin I, P32.5, PAP-II, P
- Reactivity:
- Human
- Research Area:
- Cell Biology
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 46.875 pg/mL
- Range:
- 78.125-5000 pg/mL
Description
Human ANXA4 ELISA Kit
The Human ANXA4 (Annexin A4) ELISA Kit is a reliable tool for the precise measurement of ANXA4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for various research applications. ANXA4 is a key protein involved in the regulation of inflammation, cell proliferation, and apoptosis. Dysregulation of ANXA4 has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune diseases.
Therefore, the Human ANXA4 ELISA Kit is essential for studying the role of ANXA4 in these conditions and developing potential therapeutic interventions. Overall, the Human ANXA4 ELISA Kit provides researchers with a valuable tool to better understand the function of ANXA4 and its potential implications in disease pathology.
| Product Name: | Human ANXA4 ELISA Kit |
| SKU: | HUFI01594 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 46.875 pg/mL |
| Range: | 78.125-5000 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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