The Human ANG2 (Angiopoietin-2) ELISA Kit is specifically designed for the accurate detection of Angiopoietin-2 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiopoietin-2 is a key protein involved in angiogenesis, playing a crucial role in blood vessel formation and cell proliferation. This protein is implicated in various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions. Overall, the Human ANG2 ELISA Kit provides researchers with a powerful tool for investigating the role of Angiopoietin-2 in various physiological and pathological processes, ultimately contributing to advancements in the field of biomedical research.
Product Name:
Human ANG2 (Angiopoietin 2) ELISA Kit
SKU:
HUES01295
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
