Human Cell Biology ELISA Kits 5
Human AGC (Aggrecan) ELISA Kit (HUES01524)
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- SKU:
- HUES01524
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P16112
- ELISA Type:
- Sandwich
- Synonyms:
- ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, cartilage-specific proteoglycan core protein, chondroitin sulfate proteoglycan 1
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 0.1 ng/mL
- Range:
- 0.16-10 ng/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Human AGC (Aggrecan) ELISA Kit
The Human AGC (Aggrecan) ELISA Kit is a powerful tool for the precise measurement of aggrecan levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an indispensable asset for a wide range of research studies.Aggrecan is a key component of the extracellular matrix in cartilage tissue, playing a vital role in maintaining cartilage structure and function. Changes in aggrecan levels are associated with various musculoskeletal disorders, including osteoarthritis and rheumatoid arthritis. By accurately measuring aggrecan levels, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and develop potential therapeutic strategies.Overall, the Human AGC ELISA Kit is a valuable tool for investigating the role of aggrecan in cartilage health and disease, providing researchers with the means to advance our understanding of musculoskeletal disorders and develop innovative treatment approaches.
| Product Name: | Human AGC (Aggrecan) ELISA Kit |
| SKU: | HUES01524 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 0.1 ng/mL |
| Detection range: | 0.16-10 ng/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 4 vials, 20 mL | 96T*5: 20 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
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| Uniport ID: | P16112 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human AGC in samples. No significant cross-reactivity or interference between Human AGC and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Signal Transduction,Stem Cells |
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