Human Adenylate cyclase type 1/ADCY1 ELISA Kit (HUFI01237)
SKU:
HUFI01237
UPC:
MPN:
Product Type:
ELISA Kit
Uniprot:
Q08828
ELISA Type:
Sandwich
Synonyms:
ADCY1, Adenylate cyclase type 1, Ca, 2+, calmodulin-activated adenylyl cyclase, Adenylyl cyclase 1, ATP pyrophosphate-lyase 1, Adenylate cyclase type I
The Human Adenylate Cyclase Type 1 (ADCY1) ELISA Kit is a powerful tool for the accurate measurement of ADCY1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. Adenylate Cyclase Type 1 is a key enzyme involved in cellular signaling pathways, particularly in cAMP-mediated signal transduction. Dysregulation of ADCY1 has been linked to various diseases, including metabolic disorders, cardiovascular diseases, and neurological conditions.
Therefore, measuring ADCY1 levels can provide valuable insights into these disease processes and aid in the development of potential therapeutic interventions. Overall, the Human ADCY1 ELISA Kit is an indispensable tool for researchers seeking to understand the role of ADCY1 in health and disease, offering reliable and accurate results for a wide range of experimental settings.
Product Name:
Human Adenylate cyclase type 1/ADCY1 ELISA Kit
SKU:
HUFI01237
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.469 ng/mL
Range:
0.781-50 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-105%
88-99%
81-99%
EDTA Plasma (n = 5)
87-104%
87-96%
83-99%
Heparin Plasma (n = 5)
86-102%
82-101%
82-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
93-103
99
EDTA Plasma (n = 5)
89-104
99
Heparin Plasma (n = 5)
86-104
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
system_update_altDatasheetHuman Adenylate Cyclase 1, Brain (ADCY1) ELISA KitThe Human Adenylate Cyclase 1 (Brain Adcy1) ELISA Kit is a reliable tool for the detection of Adcy1
