ADAM10, CD156c, Kuzbanian, MADM, a disintegrin and metalloprotease domain 10, AD10, ADAM 10, ADAM metallopeptidase domain 10, CD156c, CD156c antigen, CDw156, disintegrin and metalloproteinase domain-containing protein 10
ADAM Metallopeptidase Domain 10 (ADAM10) is a serine protease that catalyzes the hydrolysis of peptide bonds in peptide chains, including those in proteins, as well as some types of carbohydrates. ADAM10 plays a key role in the function of clearing amyloid-beta peptides from the body. Amyloid-beta peptides are large, toxic protein fragments that can be found in the brain tissue and spinal cord of patients with Alzheimer's disease. These protein fragments accumulate as waste products and are believed to cause inflammation and cell death.
ADAM10, CD156c, Kuzbanian, MADM, a disintegrin and metalloprotease domain 10, AD10, ADAM 10, ADAM metallopeptidase domain 10, CD156c, CD156c antigen, CDw156, disintegrin and metalloproteinase domain-containing protein 10
Detection method:
Sandwich ELISA, Double Antibody
Application:
This immunoassay kit allows for the in vitro quantitative determination of Human ADAM10 concentrations in serum plasma and other biological fluids.
Sensitivity:
37.5pg/ml
Range:
62.5-4000pg/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human ADAM10 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ADAM10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
96
EDTA plasma(n=5)
86-103
96
UFH plasma(n=5)
85-105
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ADAM10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
92-105%
87-103%
EDTA plasma(n=5)
87-101%
85-98%
84-101%
UFH plasma(n=5)
81-100%
81-100%
87-98%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala--Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like
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