Human ABAT (4-aminobutyrate aminotransferase, mitochondrial) ELISA Kit (AEFI00246)

Human ABAT (4-aminobutyrate aminotransferase, mitochondrial) ELISA Kit

The Human ABAT (4-Aminobutyrate Aminotransferase) Mitochondrial ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of ABAT levels in human samples including serum, plasma, and cell culture supernatants. This kit ensures precise and reproducible results, making it an invaluable tool for a wide range of research applications. ABAT is a key enzyme involved in the metabolism of γ-aminobutyric acid (GABA), an important neurotransmitter in the central nervous system. Dysregulation of ABAT activity has been linked to various neurological disorders such as epilepsy, schizophrenia, and Alzheimer's disease, making it a critical biomarker for studying these conditions and exploring potential treatment opportunities.

With its high specificity and ease of use, the Human ABAT Mitochondrial ELISA Kit is an essential tool for researchers looking to investigate the role of ABAT in health and disease, providing valuable insights into the pathogenesis of neurological disorders and paving the way for the development of novel therapeutic interventions.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.