GST-HRP Monoclonal Antibody [PGSTHRPSHG] (CPAB0427)

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SKU:
CPAB0427
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
GST
Host Species:
Mouse
Isotype:
IgG2b
Clone:
PGSTHRPSHG
Applications:
WB

Description

system_update_alt Datasheet
Product Name:GST-HRP Antibody
Product Sku:CPAB0427
Size:100μl
Host Species:Mouse
Immunogen:
Clone:PGSTHRPSHG.
Reactivity:-GST
Applications:Western Blot
Purification Method:
Isotype:IgG2b
Background:GST family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions.
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