Metabolism Assays

Glyoxalase I Activity Assay Kit (Colorimetric) (BA0026)

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SKU:
BA0026
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Enzyme Preparations Or Biological Samples
Research Area:
Metabolism

Description

ELISA Kit Technical ManualMSDS

Glyoxalase I Activity Assay Kit - Information

Assay Genie's Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.

Applications

For quantitative determination of glyoxalase activity. Note: use a UV plate

Glyoxalase I Activity Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 uL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Glyoxalase I Activity Assay Kit - Data Sheet
Kit Includes Assay Buffer (pH 6.6): 20 mL Substrate: 1 mL 96 well UV Titer Plate 1 Plate Cosubstrate: 1 mL
Kit Requires Perchloric Acid, pipetting devices and accessories and plate reader.
Method of Detection OD240nm
Detection Limit 4 U/L
Samples Enzyme preparations or biological samples
Species All
Protocol Length 30 min
Size 100 tests
Storage Store the plate at room temperature and other components at - 20°C
Shelf Life 12 months

More Details

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery.

Aliases for GLO1 Gene

  • Glyoxalase I
  • S-D-Lactoylglutathione Methylglyoxal Lyase
  • Glyoxalase Domain Containing 1
  • Ketone-Aldehyde Mutase
  • Methylglyoxalase
  • Aldoketomutase
  • EC 4.4.1.5
  • Glx I

Entrez Gene Summary for GLO1 Gene

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008
UniProtKB/Swiss-Prot for GLO1 Gene LGUL_HUMAN,Q04760Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathioneInvolved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.

Protein details for GLO1 Gene (UniProtKB/Swiss-Prot)

Protein Symbol:Q04760-LGUL_HUMAN
Recommended name:Lactoylglutathione lyase
Protein Accession:Q04760
Secondary Accessions:B2R6P7,B4DDV0,P78375,Q59EL0,Q5TZW3,Q96FC0,Q96J41

Protein attributes for GLO1 Gene

Size:184 amino acids
Molecular mass:20778 Da
Cofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Quaternary structure:Homodimer.
SequenceCaution:Sequence=BAD93038.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

Post-translational modifications for GLO1 Gene

  • Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.Q04760-LGUL_HUMAN
  • Glutathionylation at Cys-139 inhibits enzyme activity.Q04760-LGUL_HUMAN
  • Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B.Q04760-LGUL_HUMAN
  • Ubiquitination at Lys 44, Lys 140, and Lys 148 NX_Q04760
  • Modification sites at PhosphoSitePlus Q04760

Datasheets

ELISA Kit Technical ManualMSDS

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