Glutathione Peroxidase 4 (GPX4) Activity Assay Kit
- SKU:
- MAES0287
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Animal Tissue And Cell
- Sensitivity:
- 0.96U/L
- Range:
- 0.96-44.69U/L
Description
| Product Name: | Glutathione Peroxidase 4 (GPX4) Activity Assay Kit |
| Product Code: | MAES0287 |
| Sample Type: | Serum, Plasma, Animal Tissue And Cell |
| Assay Type: | Enzyme Activity |
| Synonyms: | GPX4 |
| Detection Instrument: | Microplate reader (340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.96U/L |
| Detection Range: | 0.96-44.69U/L |
| Inter-assay CV: | 0.4-3.4% |
| Intra-assay CV: | 1.2-.3.1% |
Glutathione peroxidase 4(GPX4) is a kind of glutathione peroxidase containing selenocysteine, which can reduce phospholipid hydroperoxide to phosphatidyl alcohol, thus protecting the cell membrane from oxidative damage. GPX4 catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents.The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Double distilled water, Normal saline(0.9% NaCl) |
| Other Instruments Required: | Incubator |
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