Glutamine Synthetase (GLUL) Polyclonal Antibody (CAB21822)

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SKU:
CAB21822
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Applications:
IF
Reactivity:
Human, Mouse, Rat
Synonyms:
GS, GLNS, PIG43, PIG59, Glutamine Synthetase (GLUL)
Frequently bought together:

Description

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Glutamine Synthetase (GLUL) Polyclonal Antibody (CAB21822)

The Glutamine Synthetase (GLUL) Polyclonal Antibody (CAB21822) is a high-quality antibody developed for reliable detection and analysis of target proteins. GLUL is an enzyme that plays a crucial role in the production of the amino acid glutamine, which is essential for protein synthesis and nitrogen metabolism in cells.This polyclonal antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. It binds to the GLUL protein, allowing for detection and analysis in a variety of cell types. This antibody is ideal for studies in metabolism, cellular signaling, and cancer research, where GLUL dysregulation has been implicated in tumor growth and progression.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Glutamine Synthetase (GLUL) Polyclonal Antibody
SKU:CAB21822
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MTTS ASSH LNKG IKQV YMSL PQGE KVQA MYIW IDGT GEGL RCKT RTLD SEPK CVEE LPEW NFDG SSTL QSEG SNSD MYLV PAAM FRDP FRKD PNKL VLCE VFKY NRRP AETN LRHT CKRI MDMV SNQH PWFG MEQE YTLM GTDG HPFG WPSN GFPG PQGP YYCG VGAD RAYG RDIV EAHY RACL YAGV KIAG TNAE VMPA QWEF QIGP CEGI SMGD HLWV ARFI LHRV CEDF GVIA TFDP KPIP GNWN GAGC HTNF STKA MREE NGLK YIEE AIEK LSKR HQYH IRAY DPKG GLDN ARRL TGFH ETSN INDF SAGV ANRS ASIR IPRT VGQE KKGY FEDR RPSA NCDP FSVT EALI RTCL LNET GDEP FQYK N
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GS, GLNS, PIG43, PIG59, Glutamine Synthetase (GLUL)
Positive Sample:Mouse brain, Rat liver, Rat brain
Cellular Localization:Cytoplasm, Mitochondrion.
Calculated MW:42kDa
Observed MW:42kDa

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID2752
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit pAb (CAB21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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