Metabolism Assays

Glucose Dehydrogenase Assay Kit (Colorimetric) (BA0024)

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SKU:: BA0024
UPC:
MPN:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Biological Samples
Research Area:: Diabetes & Obesity
Research Area:: Plant & Environmental Stress
Research Area:: Clinical Chemistry

Description
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Description

Glucose Dehydrogenase Assay Kit - Information

Assay Genie's non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Applications

For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

Glucose Dehydrogenase Assay Kit - Key Features

Fast and sensitive. Linear detection range (20 sample): 0.5 to 200 U/L for 15 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Glucose Dehydrogenase Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL Diaphorase: 120 uL NAD/MTT: 1 mL Calibrator: 1.5 mL Substrate: 1 mL
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader.
Method of Detection	OD565nm
Detection Limit	0.5 U/L
Samples	Biological samples (e.g. plasma, serum, tissue and culture media).
Species	All
Protocol Length	15 min
Size	100 tests
Shelf Life	6 months
Storage	Store all components at -20°C upon receiving

More Details

GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

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