Metabolism Assays
Glucose Assay Kit (Colorimetric) (BA0041)
- SKU:
- BA0041
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Biological, Food, Beverage
- Research Area:
- Glycolysis & Carbohydrates
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Clinical Chemistry
Description
Glucose Assay Kit (Colorimetric) (BA0041) (BA0041)
The Glucose Assay Kit (Colorimetric) (SKU: BA0041) offers a simple, direct and automation-ready method for the quantitative colorimetric determination of glucose in serum, plasma, food and beverages. Glucose is a ubiquitous fuel molecule, and its concentration is a key diagnostic parameter for many metabolic disorders, with raised levels associated with diabetes mellitus and hyperactivity of the thyroid, pituitary and adrenal glands, and reduced levels found in insulin-secreting tumours, myxoedema and hypopituitarism. The assay measures glucose directly in serum or plasma without pretreatment using an improved o-toluidine method that produces a specific colour reaction with glucose. The absorbance at 630nm is directly proportional to the glucose concentration in the sample. As little as 5 µL of sample is required, making the kit both sensitive and economical. The optimised formulation greatly enhances reagent and signal stability.
| Product Name: | Glucose Assay Kit (Colorimetric) (BA0041) |
| SKU: | BA0041 |
| Detection Method: | Colorimetric |
| Detection Range: | 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate |
| Sample Type: | Serum, plasma, food and beverages |
| Species Reactivity: | All |
| Assay Time: | 8 minutes (boiling water bath) plus 4 minutes cooling |
| Kit Size: | 100 Assays |
| Equipment Required: | Microplate reader |
| Storage: | The kit is shipped at room temperature. Store the reagent at room temperature and the standard at -20°C. |
| Shelf Life: | 12 months after receipt |
| Shipping: | Room Temperature |
This kit measures glucose directly in serum or plasma without pretreatment. The improved o-toluidine method utilises a specific colour reaction with glucose, and the absorbance at 630nm is directly proportional to glucose concentration in the sample.
- Sensitive and accurate: use as little as 5 µL of sample; linear detection range 0.7 mg/dL (39 µM) to 300 mg/dL (16.6 mM) glucose in 96-well plate
- Simple and convenient: addition of a single working reagent and incubation for 8 minutes in a boiling water bath
- Improved reagent stability with an optimised formulation
- Low interference in biological samples: no pretreatments required for serum and plasma
- Direct assays: glucose in biological samples (e.g. serum and plasma)
- Drug discovery/pharmacology: effects of drugs on glucose metabolism
- Food and beverages: glucose in food, beverages etc.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Dilute standard in distilled water as shown in the table. Set up 1.5-mL centrifuge tubes and transfer 5 µL diluted standards and samples to labelled tubes. Add 500 µL Reagent to each tube, close tightly and mix. Diluted standards may be stored at -20°C for future use. |
| 2 | Place tubes in a holder and heat in a boiling water bath or heat block for 8 minutes, then cool in a cold water bath for 4 minutes. |
| 3 | Transfer 200 µL in duplicate into a clear bottom 96-well plate, avoiding bubble formation, and read optical density at 620-650nm (peak absorbance at 630nm). |
Subtract blank OD (water, #5) from the standard OD values and plot OD against standard concentrations. Determine the slope by linear regression. Glucose concentration = (ODSAMPLE − ODBLANK) / Slope (mg/dL). Typical serum/plasma glucose values: 70-110 mg/dL. Conversion: 1 mg/dL glucose equals 55.5 µM, 0.001% or 10 ppm.
| Component | Quantity | Storage |
| Reagent | 50 mL | Room temperature |
| Standard (300 mg/dL) | 1 mL | -20°C |
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