Metabolism Assays
Glucose Assay Kit (BA0081)
- SKU:
- BA0081
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Urine, Saliva, Milk, Culture Medium, Food, Agriculture
- Research Area:
- Glycolysis & Carbohydrates
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Clinical Chemistry
Description
Glucose Assay Kit - Informatoion
Assay Genie's glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glucose concentration in the sample.Applications
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.Glucose Assay Kit - Key features
- Sensitive and accurate. Use as little as 20 samples. Linear detection range in 96-well plate: 5 to 300 (90 /dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 for fluorimetric assays.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
| Kit Includes | Assay Buffer: 10 mL Enzyme Mix: 120 mL Dye Reagent: 120 mL Standard: 1 mL 300 mg/dL Glucose |
| Kit Requires | Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader. |
| Method of Detection | OD570nm, or FL530/585nm |
| Detection Limit | 5 |
| Samples | Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc |
| Species | All |
| Protocol Length | 40 min |
| Size | 100 tests |
| Storage | Store Assay Buffer at 4°C and other components at -20°C |
| Shelf Life | 6 months |
More Details
Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery.| Neto et al. | 2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identified | bioRxiv 2023 | View Citation |
Related Products
Glucose Assay Kit III
Product Coming SoonIf you have a question, please don't hesitate to get in touch!
Glucose Assay Kit II
Product Coming SoonIf you have a question, please don't hesitate to get in touch!
Glucose Oxidase (GOD) Activity Assay Kit
OverviewProduct Name:Glucose Oxidase (GOD) Activity Assay KitProduct Code:MAES0248Assay Time:35 minAssay Type:Enzyme Activity
