GeniePlex Human Chemokine Extended Multiplex Assay (12-Plex) (HUAMPM053)

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SKU:
HUAMPM053
Product Type:
Multiplex
Reactivity:
Human
ELISA Type:
Multiplex
Analytes:
Fractalkine/CX3CL1, CCL11/Eotaxin, CXCL9/MIG, CCL17/TARC, CXCL10/IP-10, CCL22/MDC, IL-8/CXCL8, CCL2/MCP-1, CCL3/MIP-1-alpha, CCL7/MCP-3, CCL4/MIP-1-beta, CCL5/RANTES
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Description

system_update_altDatasheet
Product Name:GeniePlex Human Chemokine Extended Multiplex Assay (12-Plex) (96 Tests)
Product Code:HUAMPM053
Size:96 Tests
Reactivity:Human
Analytes (12):Fractalkine/CX3CL1, CCL11/Eotaxin, CXCL9/MIG, CCL17/TARC, CXCL10/IP-10, CCL22/MDC, IL-8/CXCL8, CCL2/MCP-1, CCL3/MIP-1-alpha, CCL7/MCP-3, CCL4/MIP-1-beta, and CCL5/RANTES
Sensitivity (LODs):Fractalkine/CX3CL1: N/A pg/mL, CCL11/Eotaxin: < 5 pg/mL, CXCL9/MIG: < 10 pg/mL, CCL17/TARC: < 5 pg/mL, CXCL10/IP-10: < 3 pg/mL, CCL22/MDC: < 5 pg/mL, IL-8/CXCL8: < 5 pg/mL, CCL2/MCP-1: < 2 pg/mL, CCL3/MIP-1-alpha: < 2 pg/mL, CCL7/MCP-3: < 2 pg/mL, CCL4/MIP-1-beta: < 5 pg/mL, CCL5/RANTES: < 5 pg/mL
Sample types:Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Assay Type: Multiplex
Detection Method:Flow Cytometry
Assay Time: 2 hours
1x Ab-conjugated beads (premixed):anti-human Fractalkine/CX3CL1, S4P1 - anti-human CCL11/Eotaxin, S4P2 - anti-human CXCL9/MIG, S4P3 - anti-human CCL17/TARC, S4P4 - anti-human CXCL10/IP-10, S4P7 - anti-human CCL22/MDC, S4P9 - anti-human IL-8/CXCL8, S5P1 - anti-human CCL2/MCP-1, S5P2 - anti-human CCL3/MIP-1-alpha, S5P3 - anti-human CCL7/MCP-3, S5P7 - anti-human CCL4/MIP-1-beta, S5P7 - anti-human CCL5/RANTES
2x Biotin-detection Ab (premixed):Biotin - anti-human Fractalkine/CX3CL1, Biotin - anti-human CCL11/Eotaxin, Biotin - anti-human CXCL9/MIG, Biotin - anti-human CCL17/TARC, Biotin - anti-human CXCL10/IP-10, Biotin - anti-human CCL22/MDC, Biotin - anti-human IL-8/CXCL8, Biotin - anti-human CCL2/MCP-1, Biotin - anti-human CCL3/MIP-1-alpha, Biotin - anti-human CCL7/MCP-3, Biotin - anti-human CCL4/MIP-1-beta, Biotin - anti-human CCL5/RANTES
Buffers:2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix- Human Chemokine Extended 12-Plex. Vials A, B and C containing lyophilized recombinant human Fractalkine/CX3CL1, CCL11/Eotaxin, CXCL9/MIG, CCL17/TARC, CXCL10/IP-10, CCL22/MDC, IL-8/CXCL8, CCL2/MCP-1, CCL3/MIP-1-alpha, CCL7/MCP-3, CCL4/MIP-1-beta, CCL5/RANTES.
LLOQs:Fractalkine/CX3CL1: N/A pg/mL, CCL11/Eotaxin: < 10 pg/mL, CXCL9/MIG: < 20 pg/mL, CCL17/TARC: < 10 pg/mL, CXCL10/IP-10: < 10 pg/mL, CCL22/MDC: < 10 pg/mL, IL-8/CXCL8: < 10 pg/mL, CCL2/MCP-1: < 5 pg/mL, CCL3/MIP-1-alpha: < 5 pg/mL, CCL7/MCP-3: < 5 pg/mL, CCL4/MIP-1-beta: < 10 pg/mL, CCL5/RANTES: < 10 pg/mL.
ULOQs:Fractalkine/CX3CL1: N/A pg/mL, CCL11/Eotaxin: > 2,000 pg/mL, CXCL9/MIG: > 5,000 pg/mL, CCL17/TARC: > 2,000 pg/mL, CXCL10/IP-10: > 2,000 pg/mL, CCL22/MDC: > 5,000 pg/mL, IL-8/CXCL8: > 5,000 pg/mL, CCL2/MCP-1: > 5,000 pg/mL, CCL3/MIP-1-alpha: > 2,000 pg/mL, CCL7/MCP-3: > 5,000 pg/mL, CCL4/MIP-1-beta: > 5,000 pg/mL, CCL5/RANTES: > 5,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: <10%
Inter-assay CV:Inter-assay CV: <20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume:15 µL/test

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

A filter plate washer is required for the following protocol. For more information on GeniePlex click here.

StepProtocol
1.Prepare the filter plate template.
2.Vortex working bead suspension for 15 seconds.
3.Add 45 µL of capture bead working suspension to each well.
4.Remove buffer in the wells by using the Filter Plate Washer connected to a vacuum source.
5.Remove buffer in the wells by using the Filter Plate Washer connected to a vacuum source.
6.Add 30 µL of CCS, SPB or TL Assay Buffer to each sample well.
7.Add 15 µL of samples to each sample well. Add 45 µL of standards to each standard well. Cover the plate with a plate seal.
8.Incubate on the shaker (set at 700 rpm) for 60 min at room temperature. Protect from light.
9.Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer.
10.Remove solutions in the wells by using the Filter Plate Washer.
11.Wash the wells three times with 100µL 1x Wash Buffer.
12.Gently tap the plate bottom onto paper towels to remove residual buffer.
13.Add 25µL of biotinylated antibody working solution to each well. Cover with plate seal.
14.Incubate on the shaker (700 rpm) for 30 min at room temperature. Protect from light.
15.Remove plate seal. Remove solutions. Wash three times with 100 µL 1x Wash Buffer.
16.Gently tap the plate bottom onto paper towels.
17.Add 25µL of streptavidin-PE working solution to each well. Cover with plate seal.
18.Incubate on the shaker (700 rpm) for 20 min at room temperature. Protect from light.
19.Remove plate seal. Remove solutions. Wash twice with 100 µL 1x Wash Buffer.
20.Add 150-300µL of 1x Reading Buffer to each well to re-suspend beads. Cover with plate seal.
21.Shake for 30 seconds at 700 rpm. Read on a flow cytometer.

GeniePlex Technology

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

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