GenieFluor Violet 450 Anti-Mouse Ly6A/E(Sca-1) Antibody [D7] (AGEL3345)

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SKU:
AGEL3345
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FCM
Reactivity:
Mouse
Host Species:
Rat
Isotype:
Rat IgG2a, k
Clone:
D7
Conjugation:
AFViolet 450

Description

Product Name:AFViolet 450 Anti-Mouse Ly6A/E(Sca-1) Antibody [D7]
Product Code:AGEL3345
Size:25ug, 100ug
Clone No:D7
Synonyms:Ly6a, Ly6, Ly-6A.2/Ly-6E.1, SCA-1, TAP
Reactivity:Mouse
Host Species:Rat
Applications:FCM
Isotype:Rat IgG2a, κ
Isotype Control:EV450 Rat IgG1, κ Isotype Control [HRPN]
Conjugation:AFViolet 450
Applications:FCM
Recommended Dilution:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Storage:Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Fluorescent Label:GenieFluor Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).
Shipping:Biological ice pack at 4°C
Form:Liquid
Gene ID:110454
Swissprot:P05533
Background Information:Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
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