Free Fatty Acids Assay Kit - Fluorometric (MAES0010)
- SKU:
- MAES0010
- Product Type:
- Assay
- Detection Method:
- Fluorometric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Animal Tissue
- Sensitivity:
- 0.58 umol/L
- Range:
- 0.58-20 umol/L
- Assay Time:
- 70 min
- Shipping:
- Gel Pack
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Clinical Chemistry
Description
| Product Name: | Free Fatty Acids Assay Kit - Fluorometric |
| Product Code: | MAES0010 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 70 min |
| Instrument: | Fluorescence Microplate Reader |
| Sensitivity: | 0.58 µmol/L |
| Detection Range: | 0.58-20 µmol/L |
| Recovery Rate: | 96 |
| Inter CV: | 5.3 |
| Inter CV: | 4.2 |
Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormone-sensitive lipase (HSL) decomposes triglycerides to produce glycerol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.
Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Lipids metabolism |
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