Flotillin 1 Polyclonal Antibody (CAB21832)

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SKU:
CAB21832
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Applications:
IHC
Reactivity:
Human, Mouse, Rat
Synonyms:
FLOT1, flotillin-1, Flotillin 1
Frequently bought together:

Description

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Flotillin 1 Polyclonal Antibody (CAB21832)

The Flotillin 1 Polyclonal Antibody (CAB21832) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, and is validated for use in Western blot applications. By binding to the flotillin-1 protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential reagent for studies in cell biology and signal transduction.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Flotillin 1 Polyclonal Antibody
SKU:CAB21832
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SEQV FKVA SSDL VNMG ISVV SYTL KDIH DDQD YLHS LGKA RTAQ VQKD ARIG EAEA KRDA GIRE AKAK QEKV SAQY LSEI EMAK AQRD YELK KAAY DIEV NTRR AQAD LAYQ LQVA KTKQ QIEE QRVQ VQVV ERAQ QVAV QEQE IARR EKEL EARV RKPA EAER YKLE RLAE AEKS QLIM QAEA EAAS VRMR GEAE AFAI GARA RAEA EQMA KKAE AFQL YQEA AQLD MLLE KLPQ VAEE ISGP LTSA NKIT LVSS GSGT MGAA KVTG EVLD ILTR LPES VERL TGVS ISQV NHKP LRTA
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FLOT1, flotillin-1, Flotillin 1
Positive Sample:Hep G2, HeLa, 293T, A549, Mouse lung, Mouse heart, Mouse brain
Cellular Localization:Cell Membrane, Endosome, Melanosome, Membrane, Peripheral Membrane Protein, Caveola.
Calculated MW:47kDa
Observed MW:Refertofigures

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID10211
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Flotillin 1 Rabbit pAb (CAB21832) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat heart using Flotillin 1 Rabbit pAb (CAB21832) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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