CYP19A (Aromatase) Activity Assay
- SKU:
- ARMA00165
- Product Type:
- Enzyme Activity Assay
- Detection Method:
- Fluorometric
- Excitation:
- 490 nm
- Size:
- 200 Reactions
Frequently bought together:
Description
system_update_altDatasheet
| Product Name: | CYP19A (Aromatase) Activity Assay |
| Product Code: | ARMA00165 |
| Product Size: | 200 Reactions |
| Detection Principle: | Fluorometric |
| Excitation: | 490 nm |
| Emission: | 535 nm |
| RUO: | Research-Use-Only |
In the presence of CYP19A and NADPH, a select substrate is dealkylated to a fluorescent product. The rate of increase in fluorescence is directly proportional to CYP19A activity.
| Plate Reader Temperature: | 37 °C |
| Data Collection Interval: | Every 30-60 seconds |
| Measurement Duration: | 30-60 minutes |
| Preincubation Time: | 15 minutes at 37 °C |
| Sample Protein Range: | 25-50 µg microsomal protein; 50-200 µg S9 or lysates |
| Standard Range: | 0-2.5 pmoles |
| Unopened Kit Storage: | -20 °C |
| Assay Buffer: | 4 °C |
| Fluorescein Standard: | 4 °C |
| Lyophilized Components: | -20 °C |
| Active Aromatase Storage: | -80 °C (loses ~10% activity per week) |
| Reconstitution: | Active Aromatase immediately before use only |
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