Cleaved Gasdermin D (N Terminal) Rabbit Monoclonal Antibody (CAB24059)

Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059)

The Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using high-quality rabbit monoclonal technology, specifically targets the N-terminal fragment of cleaved Gasdermin-D. It has been validated for use in Western blot and immunofluorescence applications, allowing for precise detection and visualization of cleaved Gasdermin-D in various cell types.Gasdermin-D is a key player in the process of pyroptosis, a programmed cell death pathway that is triggered by inflammatory signals. By targeting the N-terminal fragment of cleaved Gasdermin-D, this antibody enables researchers to investigate the mechanisms underlying pyroptosis and its role in inflammatory responses.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.

Western blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 20s.