Choline/Acetylcholine Fluorometric Assay Kit
- SKU:
- MAES0278
- Product Type:
- Assay
- Instrument:
- Fluorescence Microplate Reader
- Detection Method:
- Fluorometric
- Sample Type:
- Serum, Plasma And Animal Tissue
- Sensitivity:
- 0.06 μmol/L
- Range:
- 0.06-10 μmol/L
Description
| Product Name: | Choline/Acetylcholine Fluorometric Assay Kit |
| Product Code: | MAES0278 |
| Sample Type: | Serum, Plasma And Animal Tissue |
| Assay Type: | Quantitative |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Detection Method: | Fluorescence method |
| Sensitivity: | 0.06 μmol/L |
| Detection Range: | 0.06-10 μmol/L |
| Inter-assay CV: | 8.2-10% |
| Intra-assay CV: | 3.2-5% |
Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems. Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Incubator(37℃) |
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