Cholesterol Assay Kit (BA0064)

Cholesterol Assay Kit - Information

Assay Genie's Cholesterol Assay (Colorimetric/Fluorometric) uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to total cholesterol concentration in the sample.

Applications

For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.

Cholesterol Assay Kit - Key Features

• Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.
• Convenient. Room temperature assay. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Cholesterol Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 20 mL Enzyme Mix: 120 uL Dye Reagent: 120 mL Standard: 1 mL 300 mg/dL cholesterol
Kit Requires	Pipetting (multi-channel) devices, 96-well plate and plate reader
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	OD, FL: 1, 0.2 mg/dL
Samples	Serum, plasma etc
Species	All
Protocol Length	40 min
Size	100 tests
Storage	Store reagents at -20°C
Shelf Life	12 months

More Details

CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable.