Chicken ELISA Kits
Chicken IGFBP-3/Insulin Like Growth Factor Binding Protein 3 ELISA Kit (CHFI00029)
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- SKU:
- CHFI00029
- Product Type:
- ELISA Kit
- Uniprot:
- A6N8N6
- ELISA Type:
- Competitive
- Synonyms:
- IGFBP-3, IGFBP3, BP-53, IBP3
- Reactivity:
- Chicken
- Size:
- 48T/96T
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 3.75 ng/mL
- Range:
- 6.25-400 ng/mL
Frequently bought together:
Description
Chicken IGFBP-3/Insulin Like Growth Factor Binding Protein 3 ELISA Kit
The Chicken IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) ELISA Kit is a powerful tool for the precise measurement of IGFBP-3 levels in chicken serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers highly accurate and consistent results, making it well-suited for various research applications. IGFBP-3 is a key player in regulating the activity of insulin-like growth factors, impacting cell growth, differentiation, and survival.
Its dysregulation has been linked to various health issues, including metabolic disorders and cancer. By detecting and quantifying IGFBP-3 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.
| Product Name: | Chicken IGFBP-3/Insulin Like Growth Factor Binding Protein 3 ELISA Kit |
| SKU: | CHFI00029 |
| Reactivity: | Chicken |
| Assay Type: | Competitive ELISA, Coated with Antigen |
| Sensitivity: | 3.75 ng/mL |
| Range: | 6.25-400 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C(Sealed), Don't cryopreserve. |
| Linearity: |
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| Recovery: |
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Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice. |
| 2 | Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes. |
| 3 | Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash. |
| 4 | Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops. |
| 5 | Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately. |
| 6 | Reading: Measure absorbance at 450 nm using a microplate reader. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately. |
| Plasma | Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Cell Culture Supernatant | Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant. |
| Cell Lysate | Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant. |
| Other Sample Types | Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 5 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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