CELF4 Antibody (PACO61221)
- SKU:
- PACO61221
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | CELF4 Antibody (PACO61221) |
| Antibody SKU: | PACO61221 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human CUGBP Elav-like family member 4 protein (120-236AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61221 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. |
| Synonyms: | CUGBP Elav-like family member 4 (CELF-4) (Bruno-like protein 4) (CUG-BP- and ETR-3-like factor 4) (RNA-binding protein BRUNOL-4), CELF4, BRUNOL4 |
| UniProt Protein Function: | CELF4: RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Belongs to the CELF/BRUNOL family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: RNA splicingChromosomal Location of Human Ortholog: 18q12Cellular Component: cytoplasm; nucleusMolecular Function: mRNA binding; BRE binding; translation repressor activity, nucleic acid binding; nucleotide bindingBiological Process: positive regulation of nuclear mRNA splicing, via spliceosome; alternative nuclear mRNA splicing, via spliceosome; mRNA splice site selection; embryonic development; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of translation; regulation of alternative nuclear mRNA splicing, via spliceosome; germ cell development |
| UniProt Protein Details: | |
| NCBI Summary: | Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BZC1 |
| NCBI GenInfo Identifier: | 187761299 |
| NCBI Gene ID: | 56853 |
| NCBI Accession: | NP_001020258.1 |
| UniProt Secondary Accession: | Q9BZC1,Q59EN7, Q86XB9, Q8N2M6, Q9BQ96, Q9NR84, Q9NR85 |
| UniProt Related Accession: | Q9BZC1 |
| Molecular Weight: | 47,727 Da |
| NCBI Full Name: | CUGBP Elav-like family member 4 isoform 2 |
| NCBI Synonym Full Names: | CUGBP, Elav-like family member 4 |
| NCBI Official Symbol: | CELF4 |
| NCBI Official Synonym Symbols: | BRUNOL4; BRUNOL-4 |
| NCBI Protein Information: | CUGBP Elav-like family member 4 |
| UniProt Protein Name: | CUGBP Elav-like family member 4 |
| UniProt Synonym Protein Names: | Bruno-like protein 4; CUG-BP- and ETR-3-like factor 4; RNA-binding protein BRUNOL-4 |
| Protein Family: | CUGBP Elav-like family |
| UniProt Gene Name: | CELF4 |
| UniProt Entry Name: | CELF4_HUMAN |
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