Carboxypeptidase A2/CPA2 Recombinant Protein (RPES2616)
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- SKU:
- RPES2616
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
system_update_altDatasheet
Carboxypeptidase A2/CPA2 Recombinant Protein
Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
| Product Name: | Carboxypeptidase A2/CPA2 Recombinant Protein (RPES2616) |
| Product Code: | RPES2616 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | Carboxypeptidase A2, CPA2 |
| Accession: | NP_001860.2 |
| Sequence: | Met 1-Tyr 417 |
| Fusion tag: | C-His |
| Activity: | Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-The specific activity is >4,000 pmoles/min/µg. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 46 kDa |
| AP Mol Mass: | 46 kDa |
| Formulation: | Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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