Carboxypeptidase A1/CPA1 Recombinant Protein (RPES3995)
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- SKU:
- RPES3995
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
system_update_altDatasheet
Carboxypeptidase A1/CPA1 Recombinant Protein
Carboxypeptidase A1 ?CPA1?is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
| Product Name: | Carboxypeptidase A1/CPA1 Recombinant Protein (RPES3995) |
| Product Code: | RPES3995 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | Carboxypeptidase A1, CPA1, CPA |
| Accession: | NP_001859.1 |
| Sequence: | Met 1-Tyr 419 |
| Fusion tag: | C-His |
| Activity: | Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >3,500 pmoles/min/µg. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human CPA1 precursor (NP_001859.1) (Met 1-Tyr 419) was expressed with a C-terminal polyhistidine tag. |
| Purity: | > 97 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 47 kDa |
| AP Mol Mass: | 43 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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