Bovine Signalling ELISA Kits
Bovine Growth arrest and DNA damage-inducible protein GADD45 gamma (GADD45G) ELISA Kit
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- SKU:
- BOEB1049
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Description
| Product Name: | Bovine Growth arrest and DNA damage-inducible protein GADD45 gamma (GADD45G) ELISA Kit |
| Product Code: | BOEB1049 |
| Alias: | Growth arrest and DNA damage-inducible protein GADD45 gamma, GADD45G |
| Uniprot: | Q2KIX1 |
| Reactivity: | Bovine |
| Range: | Please contact us for more information |
| Detection Method: | Sandwich |
| Size: | 96 Assay |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | Function: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q2KIX1 |
| NCBI GenInfo Identifier: | 114052512 |
| NCBI Gene ID: | 504939 |
| NCBI Accession: | NP_001039366.1 |
| UniProt Secondary Accession: | Q2KIX1 |
| UniProt Related Accession: | Q2KIX1 |
| Molecular Weight: | 17,294 Da |
| NCBI Full Name: | growth arrest and DNA damage-inducible protein GADD45 gamma |
| NCBI Synonym Full Names: | growth arrest and DNA-damage-inducible, gamma< |
| NCBI Official Symbol: | GADD45G |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | growth arrest and DNA damage-inducible protein GADD45 gamma; growth arrest and DNA-damage-inducible protein GADD45 gamma |
| UniProt Protein Name: | Growth arrest and DNA damage-inducible protein GADD45 gamma |
| UniProt Synonym Protein Names: | |
| Protein Family: | Growth arrest and DNA damage-inducible protein |
| UniProt Gene Name: | GADD45G |
| UniProt Entry Name: | GA45G_BOVIN |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be |