Biotinylated Recombinant SARS-COV-2 Spike S1 Protein

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system_update_altDatasheetsystem_update_altMSDS

system_update_altDatasheetsystem_update_altMSDS

system_update_altDatasheetsystem_update_altMSDS

system_update_altDatasheetsystem_update_altMSDS

system_update_altDatasheetsystem_update_altMSDS

Protein Description:	Biotinylated Recombinant SARS-COV-2 SARS-COV-2 Spike S1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln14-Arg683) of SARS-COV-2 SARS-COV-2 Spike S1 fused with His tag and Avi tag at the C-terminal.
Endotoxin:	< 1 EU/μg of the protein by LAL method.
Purity:	≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:	43740568

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

	Biotinylated Recombinant SARS- COV-2 Spike S1 Protein was determined by Tris-Bis PAGE under reducing conditions.
	The purity of Biotinylated SARS- COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
	Immobilized Biotinylated SARS- COV-2 Spike S1, Hisi Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.