Neuroscience

Neuroscience

  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (STPA00015). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (STPA00015) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (STPA00015). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (STPA00015) at 1:1000.

    Anti-Ubiquitin Antibody

    Ubiquitin AntibodyUbiquitin Antibody is a rabbit polyclonal antibody directed against Ubiquitin, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and IP...
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00013). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00013) at 1:120 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Western blot analysis of Rat Tissue lysates showing detection of SOD2 (Mn) protein using Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00013). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00013) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

    Anti-SOD2 (Mn) Antibody

    SOD2 (Mn) AntibodySOD2 (Mn) Antibody is a rabbit polyclonal antibody directed against SOD2 (Mn), offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP...
  • Immunohistochemistry analysis using Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00014). Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00014) at 1:100. Left: Untreated, Right: treated with 3mmol*kg-1 NAC. Courtesy of: E. Barreiro, IMIM, Spain. Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD2 (Mn) protein using Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00014). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 µg). Lane 3: Rat Brain (15 µg). Lane 4: Rat Brain Membrane (15 µg). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD2 (Mn) Polyclonal Antibody (STPA00014) at 1:1000 for O/N at 4C.

    Anti-SOD2 (Mn) Antibody

    SOD2 (Mn) AntibodySOD2 (Mn) Antibody is a rabbit polyclonal antibody directed against SOD2 (Mn), offered by Assay Genie for research applications. Reported applications include WB, IHC, IP and ELISA...
  • Western blot analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228) showing detection of Human PSD95 protein in a transient over-expression (OE) lysate (OriGene LY419977). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-PSD95 Monoclonal Antibody, Clone B14 (STMA00228). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human PSD95 Recombinant Monoclonal [B14] (STMA00228) at 1:100 for 1 hour at RT.

    Anti-PSD-95 Antibody [B14]

    PSD-95 Antibody [B14]PSD-95 Antibody [B14] is a rabbit recombinant monoclonal antibody directed against PSD-95, offered by Assay Genie for research applications. Reported applications include WB, IHC...
  • Western blot analysis with Rabbit Anti-CASK Monoclonal Antibody, Clone PT55 (STMA00230) showing detection of Human CASK protein in a transient over-expression (OE) lysate (OriGene LY401218). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human CASK Recombinant Monoclonal [PT55] (STMA00230) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis Rabbit Anti-CASK Monoclonal Antibody, Clone PT55 (STMA00230). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human CASK Recombinant Monoclonal [PT55] (STMA00230) at 1:100 for 1 hour at RT.

    Anti-CASK Antibody [PT55]

    CASK Antibody [PT55]CASK Antibody [PT55] is a rabbit recombinant monoclonal antibody directed against CASK, offered by Assay Genie for research applications. Reported applications include WB, IHC and...
  • Dot blot analysis comparing binding affinity of STMA00226 on 10, 50, and 100 ng of Human Recombinant Alpha Synuclein Monomers, Pre-Formed Fibrils, and Dopamine-Stabilized Oligomers. Block: 5% skim milk for 1 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein Oligomer [9D2] (STMA00226) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-mouse IgG:HRP (Rockland Cat # 710-1331) at 1:4000 for 1 hour at RT. Sandwich ELISA analysis comparing detection of Human Recombinant Alpha Synuclein Monomers. The range tested for each construct was 0 to 10 ng/mL. Block: 1% BSA + 5% Sucrose in PBS pH 7.4 for 1 hour at RT. Detection antibody: HRP-conjugated and incubated for 1 hour at RT. Colour Development: TMB Substrate.

    Anti-Alpha Synuclein Oligomer Antibody [9D2]

    Alpha Synuclein Oligomer Antibody [9D2]Alpha Synuclein Oligomer Antibody [9D2] is a mouse monoclonal antibody directed against Alpha Synuclein Oligomer, offered by Assay Genie for research...
  • Western blot analysis with Rabbit Anti-CASPR2 Monoclonal Antibody, Clone FF19 (STMA00227) showing detection of Human CASPR2 protein in a transient over-expression (OE) lysate (OriGene LY402278). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human CASPR2 Recombinant Monoclonal [FF19] (STMA00227) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-CASPR2 Monoclonal Antibody, Clone FF19 (STMA00227). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human CASPR2 Recombinant Monoclonal [FF19] (STMA00227) at 1:100 for 1 hour at RT.

    Anti-CASPR2 Antibody [FF19]

    CASPR2 Antibody [FF19]CASPR2 Antibody [FF19] is a rabbit recombinant monoclonal antibody directed against CASPR2, offered by Assay Genie for research applications. Reported applications include WB,...
  • Western blot analysis comparing STMA00225 with STPA00086 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers. Block: 5% skim milk for 1/2 hour at RT. Immunohistochemistry analysis with Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (STMA00225). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (STMA00225).

    Anti-Alpha Synuclein N-terminal Antibody [11D4]

    Alpha Synuclein N-terminal Antibody [11D4]Alpha Synuclein N-terminal Antibody [11D4] is a mouse monoclonal antibody directed against Alpha Synuclein N-terminal, offered by Assay Genie for research...
  • Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00223, clone 1C1) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000. Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 2000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers. Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.

    Anti-Amyloid Beta 1-42 Oligomer Antibody [1C1]

    Amyloid Beta 1-42 Oligomer Antibody [1C1]Amyloid Beta 1-42 Oligomer Antibody [1C1] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for...
  • Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00224, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000. Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 5000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers. Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.

    Anti-Amyloid Beta 1-42 Oligomer Antibody [2F9]

    Amyloid Beta 1-42 Oligomer Antibody [2F9]Amyloid Beta 1-42 Oligomer Antibody [2F9] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for...
  • Immunohistochemistry (IHC) analysis on a temporal lobe section of a human Alzheimer’s diseased brain tissue. Block: 3% skim milk in PBST. Primary Antibody: Rabbit Anti-Amyloid Beta Oligomer Monoclonal Antibody, clone 1A2 (STMA00222) at 1:100. Secondary: Goat-anti-rabbit IgG:HRP at 1:100. Stained with DAB (1:50) and Hematoxylin (1:10). Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00222, clone 1A2) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10).

    Anti-Amyloid Beta 1-42 Oligomer Antibody [1A2]

    Amyloid Beta 1-42 Oligomer Antibody [1A2]Amyloid Beta 1-42 Oligomer Antibody [1A2] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for...
  • Dot Blot analysis of Human, Mouse, Rat Tau Peptide (pThr217) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (STMA00220). Panel A: hTau 2N4R monomer (pSer214) (2 ug). Panel B: Tau peptide (pThr217) (2 ug). Panel C: Tau peptide (non phospho) (2 ug). Load: 2ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (STMA00220) at 1:1000 for 1 hour at RT with shaking. Dot Blot analysis of Human, Mouse, Rat Tau 2N4R P301S (phospho & non-phospho) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (STMA00220). Lane 1: hTau 2N4R P301S Monomer(0.5ug). Lane 2: hTau 2N4R P301S Monomer(1.0ug). Lane 3: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(0.5ug).

    Anti-Tau Antibody (pThr217) [15B7]

    Tau Antibody (pThr217) [15B7]Tau Antibody (pThr217) [15B7] is a mouse monoclonal antibody directed against Tau Antibody (pThr217), offered by Assay Genie for research applications. Reported...